Expression Profile of PmiR-31, Novel npcRNA Of Proteus Mirabilis Under Different Growth Phases and Stress Conditions

Authors

  • Abirami Sanniraj Faculty of Applied Sciences, AIMST University, Semeling, 08100 Bedong, Kedah, MALAYSIA
  • Suresh V. Chinni Department of Biochemistry, Faculty of Medicine, Bioscience, and Nursing, MAHSA University, 42610 Jenjarom, Selangor, MALAYSIA
  • Sumitha Samuggam Faculty of Applied Sciences, AIMST University, Semeling, 08100 Bedong, Kedah, MALAYSIA. https://orcid.org/0000-0002-4259-5239
  • Eugene Boon Beng Ong Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, MALAYSIA
  • Veeranjaneya Reddy Lebaka5 Department of Microbiology, Yogi Vemana University, Kadapa AP-516005, INDIA.
  • Lee Su Yin Faculty of Applied Sciences, AIMST University, Semeling, 08100 Bedong, Kedah, MALAYSIA

DOI:

https://doi.org/10.22452/mjs.vol44sp1.4

Keywords:

Proteus mirabilis, non-protein coding RNA, PmiR-31, flagella biosynthesis

Abstract

Proteus mirabilis (P. mirabilis), known for its swarming motility, is a facultatively anaerobic, rod-shaped, Gram-negative bacterium. It forms extended swarmer cells as well as moves in a bull’s-eye formation. Proteus mirabilis significantly causes catheter-associated urinary tract infections through virulence components including flagella, fimbriae, hemolysin, urease, and proteases. Flagella-related motility allows P. mirabilis to infect the urinary system. The flagellum, composed of about 20 proteins, has a body that penetrates the cell wall, a curved hook, and a filament extending several micrometers. Previously, we knocked out PmiR-137, anticipated to modulate flhDC, which involve in the central transcriptional activator for flagellum formation and performed a differential gene expression study. We discovered npcRNA PmiR-31, expected to influence fliZ production via the TargetRNA web tool. We analyzed PmiR-31 expression under various stress and growth phases via northern blot. The PmiR-31 was highly expressed in stress conditions but not in the mutant strain. Since FliZ regulates flagella assembly, npcRNA PmiR-31 may repress fliZ translation, preventing flagella synthesis and promoting biofilm formation to evade the immune system.

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Published

12-12-2025

How to Cite

Abirami Sanniraj, Suresh V. Chinni, Sumitha Samuggam, Eugene Boon Beng Ong, Veeranjaneya Reddy Lebaka5, & Lee Su Yin. (2025). Expression Profile of PmiR-31, Novel npcRNA Of Proteus Mirabilis Under Different Growth Phases and Stress Conditions. Malaysian Journal of Science (MJS), 44(sp1), 25–29. https://doi.org/10.22452/mjs.vol44sp1.4

Issue

Vol. 44 No. sp1 (2025): Special Issue: MAHSA International Medical Sciences and Technology Conference (Mi-Med) 2024

Section

Special Issue - Mi-Med 2024

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