IN VITRO MULTIPLICATION OF TWO ECONOMICALLY IMPORTANT AND ENDANGERED MEDICINAL PLANTS - JUSTICIA GENDARUSSA BRUM AND ADENIA HONDALA (GAERTN) DE WILDE
Keywords:
In vitro, Multiple shoots, nodal explants, Justicia gendaruss, Adenia hondalaAbstract
A method for the micropropagation of Justicia gendarussa Brurn and Adenia hondala (Gaertn) de Wilde from nodal segments was developed. High percentage of proliferating microshoot cultures were obtained by placing nodal segments on Murashige and Skoog's medium supplemented with 4.44µM BAP, 8.88µM, 2.22µM, 22.2µM and 2.69µM NAA respectively (85 %). Individual shoots were excised and transferred onto half strength MS medium for rooting. The half MS medium augmented with 4.92µM IBA produced maximum number (8 & 3 respectively) and maximum percentage (80 & 70 respectively) of rooting. The plantlets could be subsequently hardened to green house conditions.
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